Effect of some mutagens for induced mutation and detected variation by SSR marker in bread wheat (Triticum aestivum L.)

Abdel-Hamed, A. A.; El-Sheihk Aly, M. M.; Saber, Sh. M.

doi:10.21608/aasj.2021.86747.1076

Effect of some mutagens for induced mutation and detected variation by SSR marker in bread wheat (Triticum aestivum L.)

Document Type : Research article

Authors

Agricultural Botany Department, Faculty of Agriculture, Al-Azhar University, 71524 Assiut, Egypt

10.21608/aasj.2021.86747.1076

Abstract

Two chemical mutagens sodium azid (SA) and hydrazine hydrate (HZ) were used to induce genetic variability for improving some morphological and agronomical traits in three bread wheat genotypes namely, Sids₁, Sids₁₂ and Giza₁₆₈. Some mutations were selected in M₁ generation plants i.e., three for Sids₁ variety )S₁ 29, S₁ 49, and S₁ 75), four for Sids₁₂ variety i.e., (S₁₂ 116, S₁₂ 161, S₁₂ 168 and Sids₁₂ 177) and one for Giza₁₆₈ (G₁₆₈ 202). Selected genotypes were grown in field experiment to obtain M₂ generation. Results showed that most agronomical traits were significantly increased in M₂ than M₁ generation plants. The highest values in selected mutated plants for 100 grain weight were 6.31 gm for S₁₂ 161 plant, 5.79 gm for G₁₆₈ 202 plant and 5.55 gm for S₁ 29 plant. Genetic variation in M₂ plants were used of evaluated by SSR molecular markers. The results showed representable the variation between treated genotypes and untreated (control) for all studied traits.

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